{ "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "FrameKey-0-0-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f742e60d-5684-4214-ba14-07b81c70e4c3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2910423, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ac4d7ef4-ac20-40ef-86a0-8bdab8dbc811", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2910892, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2bbd75e1-279c-4a0b-8683-cc52959e6395", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2911361, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "db3b4d4f-0c30-419f-adbe-212482fea522", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2911831, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "79b934a1-26d5-4efa-9635-da98f13a390a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2912300, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2bf705c5-e4a7-45ec-bfa6-6d204711740e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2912769, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dac88098-d913-4054-bc61-4dba51b740a8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2913236, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "21d5fe27-8726-4032-9b0c-64d46231ec92", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2913705, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7c42d594-02c9-416c-933b-4e2a6c5bafa3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2914175, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c7439941-f8f1-4818-896c-e3a3bca83c66", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2914645, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "46ef07f7-c8b1-461c-94f2-78737edf2d45", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2915114, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2cf1199d-c852-4b62-bfb3-8e5398a0f533", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2915583, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fdfae861-414a-49d7-a307-3e7287429a1c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2916051, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4b670519-2e4e-445c-9eb8-9418f3db631b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2916519, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9addbd10-7f0d-48e3-9be2-475bea345a2c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2916986, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ca99efb7-370f-401b-95ca-8aa1a2f805a6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2917455, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b3f5bcda-c0e5-4174-948d-31819bd91593", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2917932, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "70ca5545-eb6b-4e1d-afa1-8eb5e07f500b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2918399, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0a4e8484-75ba-41cf-a25b-b49fcc4c365c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2918869, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c7c16d66-1e1e-426c-ae45-3f2c0fbfc9a6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2919340, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cb7c030d-01aa-403d-99c4-a6c58adb1bd8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2919811, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c70ecf64-663b-4a89-82bc-48aacfaa706b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2920280, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "344843d3-7085-42ed-9432-101bda82cf96", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2920758, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3c6f3a9a-3a38-4994-84e7-5bd530cc2261", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2921226, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0b1f6fa1-2137-44e4-bf48-ad2e08e66b34", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2921693, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "68534cc6-bfb5-46c9-b90b-e6e3025a6d68", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2922162, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7c1487ee-a095-4ba4-b746-5d963cc83c0d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2922632, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "99f43ce6-76eb-4847-8bdf-66e769280c3c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2923099, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "80f0f68b-23d0-4307-a9ba-f2a41b948a89", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2923568, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1e38b57c-c586-40c9-b1ee-f20abf954eda", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2924035, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1f006ec1-f34d-4064-93ab-be2e2ebfd628", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2924503, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bb81f1a1-c4cb-4e3a-a572-b19161e126c8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2924973, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dfec9aa0-60e5-4333-a7e7-11aadc3f0e1e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2925441, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6138352d-ccd3-4cca-be82-d872087b8d05", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2925910, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a0e8adfa-ec8c-4162-bac0-2b8ecb98bc1a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2926378, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "07c91088-e78d-4c6c-afcf-b56c4edf72a6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2926845, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0d4176be-4ce5-4e29-87ab-ed0c08e3e97c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2927320, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "99f6de4f-a1e3-4e9d-9623-e4aa373e7db0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2927787, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bb4440fe-1b1f-4444-a874-24742fcafa02", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2928256, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5096b1be-2a15-495a-873e-0e1b243a8dc3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2928724, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "02bdd563-b51c-4e5a-ab63-5bc1880afff7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2929194, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8c77a611-3149-4886-a41e-48762fa79489", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2930196, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "27fe0268-ec50-401c-8566-794e5b935807", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2930443, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "85600d7d-de06-4cf7-b752-c18475b2528f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2930692, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7acb228b-c392-48e8-8c84-dee6b09ee3f9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2930942, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0eccf35f-d349-49e0-afcd-0e791606019b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2931204, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "93b61099-e121-4ea0-b9f5-ce65efa99c28", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2931453, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8511eb90-7f1c-492d-93c7-1f5e2a7ac75a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2931701, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b148937d-4609-4871-b096-1c2f11f7e7df", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2931951, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7d4c0bbd-4439-4cb2-83ca-590cbcd76c60", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2932200, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4765fcd4-9aeb-4750-8ffe-3190866487d2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2932449, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "202ce5af-78ea-4bc7-9853-25948e6c77e4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2932703, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5482e2fb-f137-42ea-90a8-8788f133251f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2932951, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "684b8d62-f9ff-439a-be42-876c8a447958", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2933200, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5e0a9c35-6b7b-4610-92c6-3726d501335a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2933450, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "99bf759a-005c-49cf-83f6-2c3e668a828d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2933700, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ac1f8a04-2374-437e-ae1b-6c66f3e34af0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2933949, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a4b883a3-56c8-4e63-836f-19d82f7f7bb8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2934208, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "32ca6c93-710b-45bb-9804-87fcdfe223c2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2934459, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "15105be1-a1f4-4707-9d36-1042b8668a46", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2934710, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3ba4315c-166d-48e0-93eb-f4dda7701dbe", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2934960, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "88a759d1-8116-460e-af70-8eecf8999371", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2935211, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "de9e0b3e-dc8b-46dc-adad-9be28aad4326", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2935459, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ded4be4e-4149-4f0c-9291-2af8fc881208", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2935719, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d920ad13-094f-48db-ab65-8ad25d3a241c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2935968, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "252b1c23-ede6-4c61-8e9a-7505e6d837e7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2936216, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8c187d4d-19a8-4820-a170-d40bf8174f44", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2936465, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2c68247c-305c-435b-8b73-2ce0f60dfeee", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2936712, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3eb08c73-3969-43fb-9539-9e85cb9d51a2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2936961, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a8b65777-4073-4fb5-9270-e806552e27bc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2937217, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e6ad0ea9-b9a7-4c66-aaa8-92028e9cb0a3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2937466, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fc03e127-fd77-475d-815a-90b0598722d7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2937714, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e847d877-bdfa-46a2-97eb-87d8d0d2bae6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2937963, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "da81fe14-a3e5-4ce9-92f0-352a75da4602", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2938213, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f9f7e3f8-b0cf-4dcf-a0cb-cb9c44e61a8a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2938463, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3174d236-aeb2-456f-9cb2-e3ad204db418", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2938711, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9d460bf2-0774-436c-9f0e-d921e0f1d106", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2938962, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fb65d50f-c3ca-474a-bfec-724bec3575bc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2939211, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d20c349c-6b6a-4411-894d-6cacdc8e6494", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2939463, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "82adcb26-b812-4cc7-ab4d-ba64bef9b857", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2939715, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d3c2b09a-f090-4a32-a670-6a979ca00bf6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2939965, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cb3e977c-847d-458f-8390-147ccee181f8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2940215, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e8091d8a-56e0-40f9-ad51-7e0d73fe3eb6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2941367, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "41be0e2f-77e0-4ae2-8b4d-9bb4b36b341f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2941816, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8da28887-f8de-4b0f-a089-6f74eb10d855", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2942265, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "80cfc55b-7d6f-463e-a9be-b97f00550eed", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2942714, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d0561fb3-1127-4ec2-a4b1-ea3ea9457400", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2943162, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "02046f9d-0479-41c6-ab5a-e1432c6a9eaa", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2943623, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9f13aa0a-e995-4683-a78d-435018b40dfd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2944071, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d11cfd21-83c8-44e0-ad55-0a18f686235c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2944519, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4d6b9ee9-4d90-44b6-bd6e-b6e9c1563029", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2944967, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "13bddd99-b651-4628-a5fd-621c806be98e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2945415, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "24337b41-af42-414f-8374-1cb71655f4c7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2945863, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bf15dc28-d822-480a-8349-261c799a9078", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2946315, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "20a255a6-233a-40e9-a545-124827795d39", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2946765, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "69fbc5b5-f532-4913-876c-edcf5264e2ce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2947214, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1f6441b7-a6f0-4cc8-9c2f-d8a89a758755", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2947663, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0417ac4e-8ba8-497e-9f07-70f958bd7006", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2948125, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "37f7f1ee-f7db-4a33-9718-f71797c69d2b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2948573, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b81edd83-b90e-44b4-9b26-f48badd16047", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2949020, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "112c45b0-836e-4129-b053-71de3a989253", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2949469, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a196e18b-e3ef-403b-b6a5-6ef9817cf1fc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2949916, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "44c40e4b-07ca-46f4-896a-ebe0b5d0e1cd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2950363, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f4ddad86-ad7a-4543-b7df-12c7e37fd29e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2950813, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "67b6a34d-6a95-4630-b0bd-6918af20f3d9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2951260, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "38c0189a-3da5-44a0-a14f-095a0ccac635", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2951711, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4bc15f6a-4491-4227-bc3d-53bf557981ae", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2952161, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d757613a-0005-4bcc-b481-fb37d6a88a98", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2952610, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "489bb394-0f0a-4424-ad2a-067f78955aa0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2953060, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "016a0cfa-15b5-4cfd-9323-73947b4e8d31", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2953509, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ea8934b6-de3f-4a23-9131-d012dbab0597", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2953957, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "07a09fbd-5ee9-470c-9b7d-dea87810573f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2954405, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a3de0264-cdd4-49e6-8eb9-bbe7bcc5453d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2954853, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2cfb0cc4-3d2f-4a9d-8f5c-7f73d2aa2d13", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2955302, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5dd32907-6212-41ca-aec5-c30101701138", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2955751, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "87b2c479-c451-44a9-86ec-0bf85f9c58d2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2956201, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "47f39628-c068-477a-acdc-a08b2fdbc873", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2956651, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f7706e77-4e0b-4457-966b-8907059a9123", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2957100, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a5ae134a-c5b7-4761-8b0c-318874e679d9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2957550, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a5e307dd-e464-48fe-b00c-986930d74312", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2957999, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "298f51b0-126e-40e2-9fd6-a89c38a02e95", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2958449, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "38f6748d-c7b1-4fc9-99a3-fd6e61e55a42", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2958899, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3290781d-9ad3-477c-8f36-a21c34df4f18", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2959362, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2606a950-c653-494c-a171-58b6b18a876f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2960235, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7ed46026-52e2-4ad5-999b-35b049afe7b1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2960822, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b490033a-324f-40ee-ae34-732707b7f855", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2961409, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9c05d848-401b-4a11-a5d9-e4fc4fcfcde4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2961997, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e3ab3e13-31b8-42a6-b0c9-f88f9b0556fd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2962584, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "da6e0a2b-07a6-4fe9-99ac-6cb7848c66c4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2963173, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:40:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "39f081ba-2661-4d09-8efa-d1e5e8a0461e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2963762, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eee2b685-782a-426f-80b4-6500f47c156d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2964353, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "774befd0-3141-4bd9-a083-7cc4f3266a4c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2964945, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5f470cec-8513-45e7-a6ea-fe64afb253ec", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2965535, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d7e1d02a-75b6-48b7-855b-e24d70733fb6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2966123, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "57cbc277-425e-404f-8771-ad12f1dd5167", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2966714, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eb93af09-6fb7-4246-945b-d8187ff3931c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2967301, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2b2ed24c-576b-4abc-8cea-e7d02d876c7c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2967888, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "93e4ab62-5bdf-48ad-b4ee-ab33ee77f20f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2968476, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6d4a9cc4-0bc7-405d-b716-40dc6b71ca38", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2969066, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eaa2885f-de84-4f25-959c-247d9978fab8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2969659, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2ddfa415-133c-4b73-8484-8fd7a25c706e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2970250, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "38da0ecb-6e77-40a1-a3d7-3b1a552cfa9c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2970841, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f276c647-0470-44f5-8655-1dcde9587bf0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2971444, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7837d4bd-9b2a-4fd6-91fd-07b07c7553fe", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2972035, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e402c1ee-35e6-4eb5-b25f-9a91b6d9e7b3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2972622, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f57b9355-5ab0-4aa1-86ba-5d2081398576", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2973210, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f07a4675-5f54-4f03-ba6d-4dab2059b7fa", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2973796, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8cd4e3a7-1bf9-4877-9812-579188d7b77d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2974385, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f0216fb3-51cf-4c19-9d2e-5b21c2e0842e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2974975, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a0e6cdb4-8194-4f3a-8dd4-2ee16d8d197b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2975567, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b3c69ada-8fae-4ea6-8d85-c09f4fc047b1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2976158, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f54095c3-4d69-42b6-acf5-2e61cbaff679", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2976748, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dd7847fa-0b5c-44e4-8056-a92220801083", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2977334, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "191bbfcd-c260-4549-a6b7-e0ccf417639c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2977922, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f2fe25bc-9bf4-4b06-add7-7e0d9d9922d0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2978509, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "31df7ee6-bc8c-4488-8bd8-34035c682725", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2979096, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "94cd208a-f287-4af8-b2db-babb9bb19320", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2979685, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f5972bec-9d99-4fc4-bd4e-db615c2d8aca", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2980273, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2780c78a-3cf9-4bf0-8429-cf2594070bf2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2980860, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cf7603e0-44fe-4b20-8de4-59c2a9174ed3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2981448, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0071d9ca-12cc-4a01-b6a2-df6a647d40a5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2982036, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0c43bc6f-a810-4901-beed-505e5a02ce05", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2982625, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ea42461d-3b44-4035-ad74-66ee3e1878af", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2983213, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "aebc4fd4-aabc-4d3f-b580-1c6e224dcf57", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2983800, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2cbf9768-2f12-43bf-a75e-983aa933e356", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2984927, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "864e3b36-db86-4b0b-990c-77b8e49fe709", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2985376, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1eeb5638-c798-4d07-a8ca-5a4bc6cd8d09", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2985824, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "adadf86f-3411-47bc-976f-0ebcb8d6c957", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2986271, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "271d0a2f-662d-44f0-ac96-5da1611b916d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2986706, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b1a87010-8395-458b-b577-3da3d21facde", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2987167, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5fc79389-1540-4332-b24f-cd7404b8f9b9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2987612, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9e7617d8-5e5c-4f33-96c9-be1aad13c27c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2988064, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2362dbda-cbb8-4bd0-970d-450fd1eb7fc2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2988529, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ab8b63f0-9bbc-4054-b84d-e5a885626472", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2988993, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f6ea5e4e-b06b-40c4-a6b1-7d414aefea48", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2989440, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "03f78662-0e9c-49eb-8775-61e91966709f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2989886, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6dca7700-1eda-48f8-8a27-be71d64c5445", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2990302, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2692f4f7-db83-4b38-81ed-b37c82c63799", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2990767, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "278ce0e0-08ec-4156-9ace-9fff161eef40", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2991215, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "17d7362a-d1e5-477d-9367-29033bdb7275", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2991648, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e4b9f470-84a7-416b-8729-3edf4cefe0b0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2992080, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dcf0fdc4-3b35-4849-828d-da36cff00688", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2992512, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e4c4fa6e-8dcd-4279-921b-f44f3f8620a2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2992958, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5690df4d-fac5-4f00-b667-aa1dcc46c07a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2993423, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a7744e58-9d4a-4c77-9552-8c2851ca55f3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2993854, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0ca25b11-e144-4879-a67d-04b619f83ccb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2994301, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d37f7bbd-3944-490c-8b36-bc7b4bfcf946", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2994734, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9f4020b4-243e-44c3-b9f6-eca1bbafdc37", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2995181, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c5add10e-22fa-48b2-9a62-512f4176729d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2995613, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "569e6839-2095-422c-9ffc-9e20bc8bc1b7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2996045, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "93b537b3-0fff-4053-90c6-9caad70f5fa5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2996478, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5dec6888-ed88-46f2-91b3-67405d75d508", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2996940, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b0b18dac-5e73-460d-b7ca-74f4313e40cd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2997374, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "96175532-f745-4677-9ffe-91268fe4f52e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2997838, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6015b0cd-6fc5-4c71-845d-59f915e6918e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2998303, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cbee97bf-01fe-44b0-b843-75305e2225e7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2998749, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dea3634d-e9a2-4554-9096-495a357127b9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2999197, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f0fc4449-e0b7-474c-ada4-8b50245ae4b3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2999644, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8f0105b3-0f35-4453-a3d0-3e5a62556a97", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3000091, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ef1a4193-6734-49bc-aa7b-8687c3e9c77e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3000540, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "73130e90-4732-4142-b407-f6261c94cd3c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3001004, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d8488921-734a-42c9-a524-fd6b630f36c5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3001452, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d9d354df-9466-49d7-a882-ea84036862a5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3001900, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ddf7efb1-e08e-47ad-873d-1e137988854d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3002349, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "31184bc7-60a7-4f05-8f75-111ffd09ce76", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 31, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 812.944 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 812.944 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 812.944 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 812.944 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 812.944 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1091.472 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1091.472 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1091.472 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1091.472 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1091.472 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1370 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1370 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1370 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1370 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1370 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1648.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1648.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1648.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1648.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1648.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54364.944, 1927.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54643.472, 1927.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 54922, 1927.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55200.528000000006, 1927.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8352.18], "XYStage": [ 55479.056000000004, 1927.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21241.944 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21241.944 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21241.944 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21241.944 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21241.944 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21520.471999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21520.471999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21520.471999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21520.471999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21520.471999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 21799 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 21799 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 21799 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 21799 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 21799 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22077.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22077.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22077.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22077.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22077.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 317.94399999999996, 22356.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 596.472, 22356.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 875, 22356.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1153.528, 22356.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8343.52], "XYStage": [ 1432.056, 22356.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "0531218d-e14f-4731-adc8-bdff3ef679f8", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-07 20:40:06 -0400", "Date": "2019-08-07", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 31, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq_2", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190807cdh exp with 3 genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9500", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3002797, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_003_001", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 1153.528, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "4", "NextFrame": 0, "ManualFocus-Position": "8350.9300", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-07 20:41:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 21520.471999999998, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "11.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" } }